PhD Defence Noémie Lloret – Københavns Universitet

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PhD Defence Noémie Lloret

Title: Label-free and fluorescence based protein arrays for biosensing

Abstract:
In the PhD thesis were investigated several protein array platforms for protein-ligand
interactions studies.
First, a study on label-free biosensing with proteins interfaced with nanowire-based field-effect transistors (NW-FET) is carried on. The experimental procedure to limit Debye-Hückel screening is discussed with emphasis on protein function, stability of the pH of the sensing buffer and the signal-to-noise ratio of the electrical response. Then, different protein arrays are investigated for membrane proteins immobilization. Two model membrane proteins are trapped in biotinylated and fluorescent amphiphilic polymers (amphipols) in order to be immobilized on surfaces for monitoring of interactions with specific antibodies by surface plasmon resonance or fluorescence microscopy.
Membrane protein arrays are developed on gold electrodes and InAs nanowire arrays for
multiplexing, proof-of-concept of membrane protein immobilization and diagnostics applications.

There will be a reception in D104 after the defense.

PhD Panel:
Supervisor: Assoc. Prof. Karen L. Martinez, Bionanotechnology and Nanomedicine Laboratory, Department of Chemistry and Nano Science Center, Copenhagen University (martinez@nano.ku.dk)
Assoc. Prof. Kenneth Lindegaard Madsen, Department of Neuroscience and Pharmacology, Copenhagen University
Assoc. Prof. Winnie Edith Svendsen, Institute for Micro- and Nanotechnology, Technical University of Denmark
Prof. Jeremy Lakey, Institute for Cell and Molecular Biosciences, The Medical School, University of Newcastle