Nano-Science Center > Research > Bionano and Nanomedicine > Martinez Group > Courses : Martinez Group

2010
Yi-Chi Liu: "Site-Specific Biofunctionalization of Nano-Objects for the Development of Fluorescent and Label-Free Biosensors"

Nathalie I. Rieben: "Development of Glutamate Biosensors using the Ionotropic Glutamate Receptor GluR2"

2008

Lars Iversen: "Surface patterning and nanowire Biosensor Construction"

2011
Nina Roberts: "Screening and Characterization of Interactions between GPCR C-terminal Tails and the PDZ Domains of NHERF-1"

Rune Frederiksen: "Development and Characterization of the InAs NWBioFET for Label-free Protein Detection"

Katrine Rønne Rostgaard: "Bimolecular Fluorescence Complementation (BiFC) based Nanowire Internalization Assay"

Randi Westh Hansen: "Investigation of two novel techniques for stabilization and immobilization of G proteincoupled receptors in aqueous solutions"

Xiaomei Li: "Towards development of a nanowire-based biosensor: cell adhesion and survival on insulators hafnium oxide and SU-8, and visualization of cytoskeleton in cells interfaced with nanowire arrays"

Lærke Münter Lassen: "Fluorescently labelled versions of the K opioid receptor for the development of biophysical assays"

2008
Thor Møller: "Development of a Surface Plasmon Resonance-based Assay for Characterizing PDZ Domain Interactions"

Trine Berthing: "Interfacing Mammalian Cells with Vertical Arrays of Inorganic Nanowires"

2010
Daniel Theodor Krommenhoek: "Optimization of a surface plasmon resonance based assay through hAGT immobilization"

2009
Nina Buch-Månson & Felix Trier: "SNAP-sgGFP Immobilization & GluR2 S1S2 Analysis -and the application of the systems in nanowire bioFET"

2008
Halfdan Kalsbeek-Hansen: "Quantification of modified surfaces using SNAP-His6-sgGFP"

Rasmus Langkllde: "Expression and Characterization of the Ligand Binding Domain of AMPA Receptor GluR2"

2007
Achebe Niels Olesen Nzulumike: "Insertion of coated glass nanoparticles in mammalian cells - fluorescence investigation"

Simon Alexander Heiberg: "Fluorescence investigation of interaction of scaffolding proteins with GPCRs in their native plasma membrane"

Alex Green Wielandt: "Fluorescence labelling of the AMPA receptor"

2006 
Jesper Langholm: "Construction, expression and study of interaction between PDZ3 and B1AR in SNAP fusion proteins"

Laerke Münter Lassen: "Fluorescence labelling of cysteine residues of the κ opioid receptor"

Mikkel Marfelt: "Immobilization and patterning of purified and non-purified proteins on solid surfaces using SNAP-tag"

Thor C. Møller: "Cloning, expression, purification and characterization of an hAGT- His10-PDZ1 fusion protein"

Trine Berthing: "Design of SNAP-tag fusion protein to facilitate immobilization of the metabotropic glutamate receptor"

 

Biochips are becoming very popular and are now adapted to routine applications in laboratories. These self-assembled lab-on-a-chip systems allows for a considerable downscaling of reagent amounts and holds the promise of high-throughput screening of e.g. blood samples for super-fast diagnosis or molecular libraries for discovery of novel drugs. 

In this course, we propose an introduction to the field, which can be of great interest to any student willing to measure molecular interactions using commercial biochips or design novel biochips. An introduction to standard modes of detection and setups for high-throughput screening will also be given.  The course will contain plenary lectures and lab exercises, so that the students will get a fundamental theoretical understanding of biochip designs and state-of-the-art in this exciting field as well as a hands-on experience with the self-assembly of such systems.

Participants: 20 students max. This multidisciplinary PhD course is of interest to students from Nanotechnology, Biology, Biochemistry, Human Biology, Biophysics, Pharmacy, Medicine.

 

Course director:
Karen L. Martinez, Associate Professor

Bionanotechnology and Nanomedicine Laboratory

Department of Neuroscience and Pharmacology & Nano-Science Center
Tel: +45 35 32 04 75, martinez@nano.ku.dK

 

 

Membrane proteins are key players in cell function and the misregulation of these proteins is identified as the underlying cause of many severe diseases.  Therefore, the majority of the commercially available drugs have membrane proteins as their targets and many resources are spent on the discovery of new drugs and on the development of nanoscale platforms for high-troughput drug screening and super-fast diagnosis.
Crucial to achieving such goals, however, is a basic understanding of the delicate nature of membrane proteins and of their complex interaction with the intra- and extracellular environment. This course will provide such a basic understanding through the exploration of the structure and function of this superfamily of proteins.  Furthermore, we will touch upon the exciting potential for the use of these natural nanomachines in bionanotechnology.
Lab exercises will cover a series of standard applications in biochemistry and cell biology for membrane protein investigations. Nanobio 1 is only required for Nano students. If you are interested in this course and did not take Nanobio 1, or need more info, contact Karen Martinez.