Talk by Dr. Ivana Vonkova
A systematic analysis of the membrane recruitment of PH domains using novel liposome microarray-based assay
Lipids have a role in virtually all biological processes, acting as structural elements, scaffolds and signaling molecules, but they are still largely under-represented in known biological networks. We have developed a liposome microarray–based assay (LiMA), a method that measures protein recruitment to membranes in a quantitative, automated, multiplexed and high-throughput manner. The lipids are present in the context of a membrane bilayer, which can be composed of a defined combination of signaling lipids and therefore mimics the complexity of biological membranes. We demonstrated the power of LiMA by revisiting the lipid-binding specificity of a large group of pleckstrin homology domains (91 in total), using liposome arrays containing 122 different types of liposomes. The dataset, consisting of results of more than 11,000 different experiments, represents one of the largest set of quantitatively measured protein-lipid interactions.
Structural & Computational Biology Unit, EMBL Heidelberg, Germany