Excitation-Emission Microscopy

In collaboration with the group of Prof. Jurgen Hauer (Technical University Munich), Prof. Giulio Cerullo and Prof. Dario Polli (Polytechnic University of Milan), we developed a single molecule fluorescence setup that allows recording 2D excitation and emission maps of single molecules. In an alternative configuration it allows measuring 2D excitation wavelength versus decay time maps. Key components on the excitation part are a continuum laser source and a newly developed common-path interferometer.

A movie explaining the concept of the common-path interferometer and the demonstration of it at the single molecule level can be found here.

Publications:

Single-molecule excitation–emission spectroscopy
Thyrhaug, E.; Krause, S.; Perri, A.; Cerullo, G.; Polli, D.; Vosch, T.; Hauer, J.
Proc Natl Acad Sci U.S.A. 2019, 116, 4064-4069.
https://www.pnas.org/content/116/10/4064.abstract

Correct version of Figure 5A

Conference talks:

https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10500/105000J/Broadband-excitation-emission-Fourier-transform-spectroscopy-of-single-molecules-at/10.1117/12.2290589.full?SSO=1

https://www.osapublishing.org/abstract.cfm?URI=FTS-2018-FT3B.5

Selected News Stories:

https://www.sciencedaily.com/releases/2019/03/190307131501.htm

https://www.eurekalert.org/pub_releases/2019-03/tuom-atl030719.php

https://phys.org/news/2019-03-approach-spectroscopy-individual-molecules.html

Acknowledgements:

Danmarks Frie Forskningsfond - Independent Research Fund Denmark
Project DFF-7014-00027