Research – University of Copenhagen

Research

* Single molecule fluoresence spectroscopy.

Our group studies the photophysical properties of organic fluorophores at the single molecule level and we also develop new fluorophores (silver nanoclusters) for single molecule spectroscopy applications. These characterizations are important to advance the field of single molecule detection and spectroscopy and have in recent years enabled subdiffraction limited fluorescence imaging. 

An example of a confocal fluorescence image showing a number of well separated individual molecules (20 by 20 micron) together with a fluorescence intensity trajectory, emission spectrum and fluorescence decay curve that can be recorded simultaneously from a single molecule.

Excitation Emission Microscopy.

With collaborators from Munich and Milan, we recently measured two-dimensional excitation-emission (2D-EEM) maps of single molecules.

* Silver nanocluster fluorescence.

In collaboration with other research groups, we explore the potential of using DNA and peptides as a scaffold for stabilizing fluorescent silver nanoclusters. Such metal clusters exhibit photophysical properties which can find applications in the biomedical field for sensing or imaging applications.

Figure: Range of different DNA stabilized silver nanoclusters.


* Time-gated, Raman and Lanthanide emission microscopy.

In collaboration with other research groups, we use our confocal microscope for diffraction limited spectroscopy and imaging of a variety of samples and materials.

Image taken from: Time-resolved confocal microscopy using lanthanide centred near-IR emission.
Liao, Z.; Tropiano, M.; Faulkner, S.; Vosch, T.; Sørensen, T.J.
RSC Advances, 2015, 5, 70282-70286.