Stamou Group – University of Copenhagen

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Stamou Group

Our lab is developing disruptive technologies to study the biophysical properties of membranes and membrane proteins on the nanoscale using fluorescence microscopy. Membrane proteins are one of the most important classes of proteins in biology comprising more than 60% of existing pharmaceutical targets. Biological membranes enable the function of membrane proteins and therefore play a key role themselves in governing a plethora of biological processes. Our work uncovers hitherto unknown nanoscale properties of membrane and membrane proteins that ultimately dictate cell behavior, and consequently provides a path towards new generations of therapeutic agents based on entirely new biological principles.

The exciting problems we are investigating are situated at the interface of biology, physics and nanotechnology, and to address them experimentally we have assembled a dynamic, interdisciplinary, group of top-tier biophysicists, biochemists, molecular biologists, and nanotechnologists. Our grants and student projects are frequently in collaboration with world-leading industrial partners including Aquaporin, Chr. Hansen, Novozymes and Novo Nordisk.

Research keywords
Nanoscale membrane biophysics, membrane curvature, single molecules, fluorescence microscopy, Ras, G protein coupled receptors, primary and secondary active transporters.

Projects

Research projects - Stamou Group

Research in our lab is focused on three main directions:

Single Transporter Activity Recordings (STARs). 
In a breakthrough paper in Science we developed a method that resolved ionic currents with a million-fold higher sensitivity than the Nobel prize awarded method of patch clamp (atto-amperes).1 This allowed us to observe for the first time the function of single transporters, revealing the existence of functional heterogeneity based on hitherto unknown off-cycle states (i.e. ultra-stable inactive and leaky states with lifetimes 105-fold longer than those of states in the classical transport cycle). The dramatic consequence of this unforeseen complexity is that many fundamental mechanistic tenets that were based on macroscopic experiments of transport will have to be revised. This truly transformative innovation is the crowing achievement of a decade of research in our lab2,3 and we believe is likely to spark off a technological and conceptual paradigm shift in the transport field.

Membrane curvature. 
Eukaryotic life is defined by the existence of intracellular membrane-bound organelles. Endomembranes are overall more curved than the plasma membrane their heterogeneous geometrical shapes however are not arbitrary, on the contrary they are so characteristic that they have become a hallmark of the different eukaryotic organelles.4 To elucidate why this phenotype is so remarkably conserved we have pioneered several high-throughput nanoscopic methods to study how membrane curvature is affecting the function(s) of membranes and membrane proteins, with an emphasis on Ras and G protein coupled receptors.5-7 Our contributions have overall helped establish the notion that the properties of biological membranes are defined equally by their lipid composition and their geometrical shape.4

Nanoscopic heterogeneity of biological membranes. 
Spatiotemporal compositional and functional heterogeneities are a hallmark of biological membranes. We are investigating the implications of these heterogeneities for biological function and also exploit them for technological applications. For example, with high-throughput single proteoliposome measurements we quantified the composition of single proteoliposomes revealing dramatic heterogeneities.8 As we showed, compositional heterogeneities can severely skew ensemble-average proteoliposome measurements however, if averaging is avoided such heterogeneities can be exploited to enable high-content screens that enable a dramatic reduction in protein consumption (~billion-fold) as compared to conventional assays.8 We are currently extending this project to investigate nanoscopic compositional and functional heterogeneities of G protein coupled receptors in live cells.

 References

  1. Science, 2016. 351 (6280): p. 1469-1473        
    Direct observation of proton pumping by a eukaryotic P-type ATPase       
    Salome Veshaguri, Sune M. Christensen, Gerdi C. Kemmer, Mads P. Møller, Garima Ghale, Christina Lohr, Andreas L.Christensen, Bo H. Justesen, Ida L. Jørgensen, Jürgen Schiller, Nikos S. Hatzakis, Michael Grabe, Thomas Günther Pomorski, Dimitrios Stamou                  
  2. Proceedings of the National Academy of Sciences. 2009. 106 (30): p. 12341
    Quantification of nano-scale intermembrane contact areas using fluorescence resonance energy transfer.                 
    Poul Martin Bendix, M. S. Pedersen and Dimitrios Stamou.
  3. Nature Nanotechnology, 2012. 7 (1): p. 51–55                   
    Mixing sub-attolitre volumes in a quantitative and highly parallel manner with soft matter nanofluidics.          
    S. M. Christensen; P.Y. Bolinger; N.S. Hatzakis; M.W. Mortensen and Dimitrios Stamou
  4. Nature Chemical Biology, 2015. 11 (11): p. 822-825          
    Membrane curvature bends the laws of physics and chemistry                    
    Lars Iversen, Signe Mathiasen, Jannik Bruun Larsen, Dimitrios Stamou
  5. Nature Chemical Biology, 2009. 5 (11): p. 835                   
    How Curved Membranes Recognize Amphipathic Helices and Protein Anchoring Motifs.                   
    N. S. Hatzakis, V. K. Bhatia, J. Larsen, K. L. Madsen, P. Y. Bolinger, A. H. Kunding, J. Castillo, U. Gether, P. Hedegård and Dimitrios Stamou.
  6. Nature Chemical Biology, 2015. 11 (3): p. 192-194            
    Front Cover Page 
    Membrane curvature enables N-Ras lipid anchor sorting to liquid-ordered membrane phases             
    Jannik Bruun Larsen, Martin Borch Jensen, Vikram K. Bhatia, Søren L. Pedersen, Thomas Bjørnholm, Lars Iversen, Mark Uline, Igal Szleifer, Knud J. Jensen, Nikos S. Hatzakis and Dimitrios Stamou
  7. Nature Chemical Biology, 2017. (in press)    
    Membrane curvature regulates sorting of GPCRs within the plasma membrane of living cells in a ligand-specific manner                
    Kadla R. Rosholm, Natascha Leijnse, Anna Mantsiou, Vadym Tkach, Søren L. Pedersen, Volker F. Wirth, Lene B. Oddershede, Knud J. Jensen, Karen L. Martinez, Nikos S. Hatzakis, Poul Martin Bendix, Andrew Callan-Jones and Dimitrios Stamou
  8. Nature Methods, 2014. 11 (9): p. 931-934     
    Nanoscale high content analysis using compositional heterogeneities of single proteoliposomes         
    Signe Mathiasen, Sune M. Christensen,, Juan Jose Fung, Soren G. F. Rasmussen, Jonathan F. Fay, Sune K. Joergensen, Salome Veshaguri, David L. Farrens, Maria Byrne, Brian Kobilka, Dimitrios Stamou

Publications

Publications - Stamou Group

Selected recent publications

  1. Nature Chemical Biology, 2017. (in press)    
    Membrane curvature regulates sorting of GPCRs within the plasma membrane of living cells in a ligand-specific manner                
    Kadla R. Rosholm, Natascha Leijnse, Anna Mantsiou, Vadym Tkach, Søren L. Pedersen, Volker F. Wirth, Lene B. Oddershede, Knud J. Jensen, Karen L. Martinez, Nikos S. Hatzakis, Poul Martin Bendix, Andrew Callan-Jones and Dimitrios Stamou
  2. Science, 2016. 351 (6280): p. 1469-1473        
    Direct observation of proton pumping by a eukaryotic P-type ATPase       
    Salome Veshaguri, Sune M. Christensen, Gerdi C. Kemmer, Mads P. Møller, Garima Ghale, Christina Lohr, Andreas L.Christensen, Bo H. Justesen, Ida L. Jørgensen, Jürgen Schiller, Nikos S. Hatzakis, Michael Grabe, Thomas Günther Pomorski, Dimitrios Stamou                  
  3. Nature Chemical Biology, 2015. 11 (11): p. 822-825          
    Membrane curvature bends the laws of physics and chemistry                    
    Lars Iversen, Signe Mathiasen, Jannik Bruun Larsen, Dimitrios Stamou
  4. Nature Chemical Biology, 2015. 11 (3): p. 192-194            
    Front Cover Page 
    Membrane curvature enables N-Ras lipid anchor sorting to liquid-ordered membrane phases             
    Jannik Bruun Larsen, Martin Borch Jensen, Vikram K. Bhatia, Søren L. Pedersen, Thomas Bjørnholm, Lars Iversen, Mark Uline, Igal Szleifer, Knud J. Jensen, Nikos S. Hatzakis and Dimitrios Stamou
  5. Nature Methods, 2014. 11 (9): p. 931-934     
    Nanoscale high content analysis using compositional heterogeneities of single proteoliposomes         
    Signe Mathiasen, Sune M. Christensen,, Juan Jose Fung, Soren G. F. Rasmussen, Jonathan F. Fay, Sune K. Joergensen, Salome Veshaguri, David L. Farrens, Maria Byrne, Brian Kobilka, Dimitrios Stamou
  6. Science, 2014, 345 (6192): p. 50-54                
    Single molecule analysis of Ras activation by SOS reveals allosteric regulation via altered fluctuation dynamics                 
    L. Iversen, H.-L. Tu, W.-C. Lin, S. M. Christensen, S. M. Abel, J. Iwig, H.-J. Wu, J. Gureasko, C. Rhodes, R. S. Petit, S. D. Hansen, P. Thill, C.-H. Yu, D. Stamou, A. K. Chakraborty, J. Kuriyan, J. T. Groves.                  
  7. Nature Nanotechnology, 2012. 7 (1): p. 51–55                   
    Mixing sub-attolitre volumes in a quantitative and highly parallel manner with soft matter nanofluidics.          
    S. M. Christensen; P.Y. Bolinger; N.S. Hatzakis; M.W. Mortensen and Dimitrios Stamou
  8. Nature Chemical Biology, 2009. 5 (11): p. 835                   
    How Curved Membranes Recognize Amphipathic Helices and Protein Anchoring Motifs.                   
    N. S. Hatzakis, V. K. Bhatia, J. Larsen, K. L. Madsen, P. Y. Bolinger, A. H. Kunding, J. Castillo, U. Gether, P. Hedegård and Dimitrios Stamou.

Selected Publications

Science, 2016. 351 (6280): p. 1469-1473 
Direct observation of proton pumping by a eukaryotic P-type ATPase           
Salome Veshaguri, Sune M. Christensen, Gerdi C. Kemmer, Mads P. Møller, Garima Ghale, Christina Lohr, Andreas L.Christensen, Bo H. Justesen, Ida L. Jørgensen, Jürgen Schiller, Nikos S. Hatzakis, Michael Grabe, Thomas Günther Pomorski, Dimitrios Stamou

Nature Chemical Biology, 2015. 11 (11): p. 822-825 
Invited Review Commentary for celebrating the 10th anniversary of Nature Chemical Biology Membrane curvature bends the laws of physics and chemistry 
Lars Iversen, Signe Mathiasen, Jannik Bruun Larsen, Dimitrios Stamou  

Nature Chemical Biology, 2015. 11 (3): p. 192-194 (Front cover page)
                
Membrane curvature enables N-Ras lipid anchor sorting to liquid-ordered membrane phases               
J.B. Larsen, M.B. Jensen, V.K. Bhatia, S.L. Pedersen, T. Bjørnholm, L. Iversen, M. Uline, I. Szleifer, K.J. Jensen, N.S. Hatzakis and D. Stamou


Nature Methods, 2014. 11 (9): p. 931-934
Nanoscale high content analysis using compositional heterogeneities of single proteoliposomes       
S. Mathiasen, S.M. Christensen,, J.J. Fung, S.G.F. Rasmussen, J.F. Fay, S.K. Joergensen, S. Veshaguri, D.L. Farrens, M. Byrne, B. Kobilka and D. Stamou

Science, 2014. 345 (6192): p. 50-54
Single molecule analysis of Ras activation by SOS reveals allosteric regulation via altered fluctuation dynamics                
L. Iversen, H.-L. Tu, W.-C. Lin, S. M. Christensen, S. M. Abel, J. Iwig, H.-J. Wu, J. Gureasko, C. Rhodes, R. S. Petit, S. D. Hansen, P. Thill, C.-H. Yu, D. Stamou, A. K. Chakraborty, J. Kuriyan and J. T. Groves.

Nature Nanotechnology, 2012, 7 (1): p. 51–55  
Mixing sub-attolitre volumes in a quantitative and highly parallel manner with soft matter nanofluidics. S.M. Christensen, P.Y. Bolinger, N.S. Hatzakis, M.W. Mortensen and D. Stamou

Nature Chemical Biology, 2009. 5 (11): p. 835 
How Curved Membranes Recognize Amphipathic Helices and Protein Anchoring Motifs. N.S. Hatzakis, V.K. Bhatia, J. Larsen, K.L. Madsen, P.Y. Bolinger, A.H. Kunding, J. Castillo, U. Gether, P. Hedegård and D. Stamou

Proceedings of the National Academy of Sciences. 2009. 106 (30): p. 12341
Quantification of nano-scale intermembrane contact areas using fluorescence resonance energy transfer. P.M. Bendix, M.S. Pedersen and D. Stamou

Publications

2016

Science, 2016. 351 (6280): p. 1469-1473 
Direct observation of proton pumping by a eukaryotic P-type ATPase           
Salome Veshaguri, Sune M. Christensen, Gerdi C. Kemmer, Mads P. Møller, Garima Ghale, Christina Lohr, Andreas L.Christensen, Bo H. Justesen, Ida L. Jørgensen, Jürgen Schiller, Nikos S. Hatzakis, Michael Grabe, Thomas Günther Pomorski, Dimitrios Stamou

2015

Nature Chemical Biology, 2015. 11 (11): p. 822-825 
Invited Review Commentary for celebrating the 10th anniversary of Nature Chemical Biology Membrane curvature bends the laws of physics and chemistry 
Lars Iversen, Signe Mathiasen, Jannik Bruun Larsen, Dimitrios Stamou 

Devlopmental Cell, 2015. 33 (2): p. 163-175 CALM Regulates Clathrin-Coated Vesicle Size and Maturation by Dirctly Sensing and Driving Membrane Curvature S.E. Miller, S. Mathiasen, N.A. Bright, F. Pierre, B.T. Kelly, N. Kladt, A. Schauss, C.J. Merrifield, D. Stamou, S. Honing and D.J. Owen.

Nature Chemical Biology, 2015. 11 (3): p. 192-194 (Front cover page)                 

Membrane curvature enables N-Ras lipid anchor sorting to liquid-ordered membrane phases.
J.B. Larsen, M.B. Jensen, V.K. Bhatia, S.L. Pedersen, T. Bjørnholm, L. Iversen, M. Uline, I. Szleifer, K.J. Jensen, N.S. Hatzakis and D. Stamou

Small, 2015. 11 (29): p. 3550-3555. Interferometric Detection of Single Gold Nanoparticles Calibrated against TEM Siza Distributions. L. Zhang, S.M. Christensen, P.M. Bendix, V.K. Bhatia, S. Loft and D. stamou

2014

Nature Methods, 2014. 11 (9): p. 931-934
Nanoscale high content analysis using compositional heterogeneities of single proteoliposomesS. Mathiasen, S.M. Christensen,, J.J. Fung, S.G.F. Rasmussen, J.F. Fay, S.K. Joergensen, S. Veshaguri, D.L. Farrens, M. Byrne, B. Kobilka and D. Stamou

Science, 2014. 345 (6192): p. 50-54
Single molecule analysis of Ras activation by SOS reveals allosteric regulation via altered fluctuation dynamics. L. Iversen, H.-L. Tu, W.-C. Lin, S.M. Christensen, S.M. Abel, J. Iwig, H.-J. Wu, J. Gureasko, C. Rhodes, R.S. Petit, S.D. Hansen, P. Thill, C.-H. Yu, D. Stamou, A.K. Chakraborty, J. Kuriyan and J.T. Groves.                                                 

Biophysical Journal, 2014. 106 (1): p. 201-209
Geometrical membrane curvature as an allosteric regulator of membrane protein structure and function. A. Tonnesen, S.M. Christensen, V. Tkach and D. Stamou.   

ACS Chemical Biology, 2014. 9 (3): p. 630-634             
Single molecule activity measurements of cytochrome P450 oxidoreductase reveal the existence of two discrete functional states. T. Laursen, A. Singha, N. Rantzau, M. Tutkus, J. Borch, P. Hedegård, D. Stamou, B.L. Møller and N.S Hatzakis

2013

Lab on a chip, 2013. 13 (18): p. 3613-3625
Single vesicle biochips for ultra-miniaturized nanoscale fluidics and single molecule bioscience. A.L Christensen, C. Lohr, S.M Christensen and D. Stamou       
– Selected for the Top 10% web collection by the Editors
– Selected as a HOT article

Cell reports, 2013, 4 (6): p. 1213-1223
Membrane-sculpting Bin-Amphiphysin-Rvs (BAR) domains generate stable lipid microdomains. H. Zhao, A. Michelot, E.V. Koskela, V. Tkach, D. Stamou, D.G. Drubin and P. Lappalainen

PLOS Biology, 2013. 11 (4): p. e1001542
PICK1 Deficiency Impairs Secretory Vesicle Biogenesis and Leads to Growth Retardation and Decreased Glucose Tolerance. B. Holst, K.L Madsen, A.M Jansen, C. Jin, M. Rickhag, V.K Lund, M. Jensen, V. Bhatia, G. Sørensen, A.N Madsen, Z. Xue, S.K Møller, D. Woldbye, K. Qvortrup, R. Huganir, D. Stamou, O. Kjærulff and U. Gether

Scientific Reports, 2013. 3: p.1565
FBAR Syndapin 1 recognizes and stabilizes highly curved tubular membranes in a concentration dependent mannerP. Ramesh, Y.F. Baroji, S. Nader S. Reihani, D. Stamou, L.B. Oddershede and P.M. Bendix

2012

Soft Matters (2012), 8(20):5658-5665
”Compositionand structure of mixed phospholipid supported bilayers formed by POPC and DPPC” A.K.L. Åkesson, T.K. Lind, N. Ehrlich, D. Stamou, H. Wacklin and M.G. Cardenas

Soft Matters (2012), 8(34):8972-8980
“Induced dye leakage by PANAM G6 does not imply dendrimer entry into vesicile lumen”A.K.L. Åkesson, C.V. Lundgaard, N. Ehrlich, T. Günter-Pomorski, D. Stamou and M.G. Cardenas

Journal of the American Chemical Society (2012), 134(22):9296-9302
"Single enzyme studies reveal the existence of discrete functional states for monomeric enzymes and how they are “selected” upon allostric regulation" N. Hatzakis, L. Wei, S.K. Jørgensen, A.H. Makarov, M. Skjot, A. Svendsen, P. Hedegård and D. Stamou

Journal of Biological Chemistry (2012), 287(41):3496-3403
“Monitoring shifts in the conformation equilibrium of the membrane protein cytochrome P450 reductase (POR) in nanodiscs” M.H. Wadsäter, T. Laursen, A. Singha, N. Hatzakis,D. Stamou, R. Barker, K. Mortensen, R.K. feidenhans´l, B.L. Møller and M.G. Cardenas

Journal of Peptide Science (2012), 18(9):579-587
“Improving membrane binding as a design strategy for amphipathic peptide hormones: 2-helix variants of PYY3-36” S.L. Pedersen, V.K. Bhatia, S. Jurt, J.K. Paulsson, M.H. Pedersen, R. Jørgensen, B. Holst, D. Stamou, N. Vrang, O. Zerbe and K.J. Jensen

Journal of the American Chemical Society (2012), 134(4):1918-1921 
Influence of the preparation route on the supramolecular organization of lipids in a vesicular system. E. Elizondo, J. Larsen, Jannik, N. Hatzakis, I. Cabrera, J. Veciana, D. Stamou, N. Ventosa

EMBO Journal (2012), 31(23):4466-4480
“Molecular basis for SNX-BAR-mediated assembly of distinct endosomal sorting tubules”J.R.T. van Weering, R.B. Sessions, C.J. Traer, D.P. Kloer, V.K. Bhatia, D. Stamou, S.R. Carlsson, J.H. Hurley and P.J. Cullen

Nature Nanotechnology (2012), 7:51-55
"Mixing subattolitre volumes in a quantitative and highly parallel manner with soft matter nanofluidics” S.M. Christensen, P-Y. Bollinger, M.W. Mortensen and D. Stamou
Also Highlighted in a News and Views Article in Nature Nanotechnology (2012), 7(1): 6-7

2011

Biophysical Journal (2011), 101(11):2693-2703  
Intermembrane docking reactions are regulated by membrane curvature .
A.H. Kunding, M.W. Mortensen, S.M. Christensen, V.K. Bhatia, I. Makarov, R. Metzler and D. Stamou

Analytical Chemistry (2011), 83(21):8169-8176  
Fluorescence anisotropy based single liposome assay to measure molecule-membrane interaction. N. Ehrlich, A. Christensen and D. Stamou

Journal of Neuroscience (2011), 31(49):18149-18154
Synapsin I senses membrane curvature by an amphipathic lipid packing sensor motif.
L. Krabben, A. Fassio, V. Bhatia, A. Pechstein, F. Onofri, M. Fadda, M. Messa, Y. Rao, O. Shupliakov, D. Stamou, F. Benfenati, and V. Haucke

Journal of Biological Chemistry (2011), 286:42603-42614
Membrane Curvature Sensing by Amphipathic Helices: A single liposome study using -synuclein and annexin B12. M.B. Jensen, V.K. Bhatia, C.C. Jao, J.E. Rasmussen, S.L. Pedersen, K.J. Jensen, R. Langen and D. Stamou

Journal of the American Chemical Society (2011), 133(28):10685  
Observation of inhomogeneity in the lipid composition of individual nanoscale liposomes.
J. Larsen, N.S. Hatzakis and D. Stamou

Langmuir (2011), 27(3):866
Monitoring Aggregation of Single Casein Micelles using Fluorescence Microscopy. 
J. Bomholt, K. Moth-Poulsen, M. Harboe, A.O. Karlson, K.B. Qvist, T. Bjørnholm andD.G. Stamou

Biophysical Journal (2011), 100(4):957
Single Vesicle Assaying of SNARE-Synaptotagmin Driven Fusion Reveals Fast and Slow Modes of Both Docking and Fusion and Intra-sample Heterogeneity. 
S.M. Christensen, M.W. Mortensen and D.G. Stamou

Nanoletters (2011), 11:888
Heat Profiling of 3D Optically Trapped Gold Nanoparticles using Vesicle Cargo Release. 
A. Kyrsting, P.M. Bendix, D.G. Stamou and L.B. Oddershede

Journal of Structural Biology (2011), 174:11-22
A structural analysis of M protein in coronavirus assembly and morphology.
B.W. Neuman, G. Kiss, A.H. Kunding, D. Bhella, M.F. Baksh, S. Connelly, B. Droese, J.P. Klaus, S. Makino, S.G. Sawicki, S.G. Siddell, D.G. Stamou, I.A. Wilson, P. Kuhn and M.J. Buchmeier

2010

Sensors (2010), 10:11352 invited Review
Sensing-Applications of Surface-Based Single Vesicle Arrays. 
S.M. Christensen and D.G. Stamou

Biosensors and Bioelectronics (2010), 26:1528-1535
Fully integrated monolithic optoelectronic transducer for real-time protein and DNA detection: The NEMOSLAB approach. K. Misiakos, P.S. Petrou, S.E. Kakabakos, D. Yannoukakos, H. Contopanagos, T. Knoll, T. Velten, M. DeFazio, L. Schiavo, M. Passamano, D. Stamou and G. Nounesis

Journal of Biological Chemistry (2010), 285(42):32486   
Membrane curvature induction and tubulation is a common feature of synucleins and apolipoproteins. J. Varkey, J.M. Isas, N. Mizuno, M.B. Jensen, V.K. Bhatia, C.C. Jao, J. Petrlova, J. Voss, D. Stamou, A.C. Steven and R. Langen

Journal of Nanomedicine (2010), 6:504 
Perspectives David versus Goliath. J. Mollenhauer, D. Stamou, A. Flyvbjerg, J. Wengel, U. Gether, J. Kjems, T. Bjørnholm and F. Besenbacher

Bioconjugate Chemistry (2010), 21:1056 
Optically Induced Linking of Protein and Nanoparticles to Gold Surfaces. 
K. Moth-Poulsen, V. Kofod-Hansen, F.S. Kamounah, N.S. Hatzakis, D.G. Stamou, K. Schaumburg and J.B. Christensen

Biophysical Journal (2010), 98:1873 
Influence of lipid heterogeneity and phase behavior on phospholipase A2 action at the single molecule level. M. Gudmand, S. Rocha, N.S. Hatzakis, K. Peneva, K. Mullen, D. Stamou, H. Uji-I, J. Hofkens, T. Heimburg and T. Bjørnholm

FEBS Letters (2010), 584: p. 1848 invited Review 
BAR Domains, Amphipathic Helices and Membrane-Anchored Proteins use the same mechanism to sense membrane curvature. K.L. Madsen, V.K. Bhatia, U. Gether and D. Stamou

Seminars in Cell and Developmental Biology (2010), 21:381 invited Review 
A Unifying Mechanism Accounts for Sensing of Membrane Curvature by BAR domains, Amphipathic Helices and Membrane-anchored proteins. V.K. Bhatia, N.S. Hatzakis andD. Stamou

2009-1997

Nature Chemical Biology (2009), 5(11):835 
How Curved Membranes Recognize Amphipathic Helices and Protein Anchoring Motifs.
N.S. Hatzakis, V.K. Bhatia, J. Larsen, K.L. Madsen, P.Y. Bolinger, A.H. Kunding, J. Castillo, U. Gether, P. Hedegård and D. Stamou.
Also Highlighted in a news and views article in Nature Chemical Biology (2009), 5 (11): p. 783Highlighted in a research Highlights Article in Lipid Maps-NatureCited at Faculty of 1000 Biologyhttp://www.f1000biology./com/article/id/1248956/evaluation. Press release has been publiches in more than 50 independent news websites

EMBO Journal (2009), 28(21):3303
Amphipathic motifs in BAR domains are essential for membrane curvature sensing. 
V.K. Bhatia, K.L. Madsen, P.Y. Bolinger, P. Hedegård, U. Gether and D. Stamou

Proceedings of the National Academy of Sciences  (2009), 106(30):12341 
Quantification of nano-scale intermembrane contact areas using fluorescence resonance energy transfer. P.M. Bendix, M.S. Pedersen and D. Stamou
Also Highlighted in a News and Views Article in Nanomedicine (2009), 4(7):699 and Highlighted in a News and Views Article in TRAC-Trends in Analytical Chemistry (2009), 28(9):7

Langmuir (2009), 25(21):12819 
Sub-Micron Patterning of Alkanethiol Self-Assembled Monolayers on Gold Using Laser Beam Irradiation. L. Iversen, O. Younes-Metzler, K. Martinez and D. Stamou

Methods in Enzymology Liposomes (2009), Book Series Methods in Enzymology, Published in 2009, Invited contribution, 465:143
Constructing size distributions of liposomes from single-object fluorescence measurements. C. Lohr, A.H. Kunding, V.K. Bhatia and D. Stamou

Journal of the American Chemical Society (2008), 130(44):14372
Encapsulation efficiency measured on single small unilamellar vesicles. B. Lohse, P-Y. Bolinger and D. Stamou

Angewandte Chemie-International Edition (2008), 47(30):5544
An Integrated Self-Assembled Nanofluidic System for Controlled Biological Chemistries. 
P.Y. Bolinger, D. Stamou and H. Vogel

Biophysical Journal (2008), 95(3):1176-1188 
A Fluorescence-Based Technique to Construct Size Distributions from Single Object Measurements, Application to the Extrusion of Lipid Vesicles. A.H. Kunding, M.W. Mortensen, S.M. Christensen and D. Stamou

Traffic (2008), 9(8):1327-1343 
Membrane localization is Critical for Activation on the PICK1 BAR domain. 
K.L. Madsen, J. Eriksen, L. Milan-Lobo, D. Han, U. Henriksen, V. Bhatia, D. Stamou, H.H. Sitte, H.T. McMahon, H.A. Weinstein and U. Gether

Langmuir (2008), 24(12):6375 
Templated Protein Assembly on Micro-Contact Printed Surface Patterns - Use of the SNAP-tag Protein Functionality. L. Iversen, N. Cherouati, T. Berthing, D. Stamou and K.L Martinez


Soft Matter, Cover Page Article (2007), 3(7):828 invited review
Surface-based lipid vesicle reactor systems: fabrication and applications.
S.M. Christensen and D. Stamou

Journal of the American Chemical Society (2006), 128(35):11328 
Subnanometer actuation of a tethered lipid bilayer monitored with fluorescence resonance energy transfer. A. Kunding and D. Stamou

Journal of Magnetic Resonance (2006), 178(1):96 
Sample patterning on NMR surface microcoils. K. Ehrmann, M. Gersbach, P. Pascoal, F. Vincent, C. Massin, D. Stamou, P.A. Besse, H. Vogel and R.S. Popovic

Angewandte Chemie-International Edition, Cover Page Article (2005),44(31):4957 
Synthesis of nanoscopic optical fibers using lipid membranes as templates .
G. Gopalakrishnan, J.M. Segura, D. Stamou, U. Gaillard, M. Gjoni, R. Hovius, K.J. Schenk, P.A. Stadelmann and H. Vogel

Langmuir (2004), 20(10):3828 
Highly fluorescent streptavidin-coated CdSe nanoparticles: Preparation in water, characterization, and micropatterning. M. Baumle, D. Stamou, J.M. Segura, R. Hovius and H. Vogel

Journal of the American Chemical Society (2004), 126(28):8594 
Integrated nanoreactor systems: Triggering the release and mixing of compounds inside single vesicles. P.Y. Bolinger, D. Stamou and H. Vogel

Langmuir (2004), 20(9):3495 
Site-directed molecular assembly on templates structured with electron-beam lithography.
D. Stamou, C. Musil, W.P. Ulrich, K. Leufgen, C. Padeste, C. David, J. Gobrecht, C. Duschl and H. Vogel

Angewandte Chemie-International Edition, Cover Page Article (2003),42(45):5580 
Self-assembled microarrays of attoliter molecular vessels. D. Stamou, C. Duschl, E. Delamarche and H. Vogel

Physical Review (2000), 62(4):5263 
Long-range attraction between colloidal spheres at the air-water interface: The consequence of an irregular meniscus. D. Stamou, C. Duschl and D. Johannsmann

Science (1998), 280(5361):273 
Friction anisotropy and asymmetry of a compliant monolayer induced by a small molecular tilt. M. Liley, D. Gourdon, D. Stamou, U. Meseth, T.M. Fischer, C. Lautz, H. Stahlberg, H. Vogel, N.A. Burnham and C. Duschl

Tetrahedron (1998), 54(15):3725 
Functionalisation of gold surfaces via topological templates. L. Scheibler, P. Dumy, D. Stamou, C. Duschl, H. Vogel and M. Mutter

Polymer Bulletin (1998), 40(2-3):151 
Self-assembling functionalized templates in biosensor technology. L. Scheibler, P. Dumy, D. Stamou, C. Duschl, H. Vogel and M. Mutter

Tribology Letters (1997), 3:317 
The dependence of friction anisotropies on the molecular organisation of LB flims as observed by AFM. D. Gourdon, N.A. Burnham, A. Kulik, E. Dupas, F. Oulevey, G. Gremaud, D. Stamou, M. Liley, Z. Dienes, H. Vogel and C. Duschl

Langmuir (1997), 13(9):2425 
Uniformly flat gold surfaces: Imaging the domain structure of organic monolayers using scanning force microscopy. D. Stamou, D. Gourdon, M. Liley, N.A. Burnham, A. Kulik, H. Vogel and C. Duschl

Funding

Stamou Group Funding Sources

The Stamou Group would like to acknowledge financial support from the following sources:

2010-2015
Lundbeck Foundation Center for Biomembrane in Nanomedicine
34 million DK
The Lundbeck Foundation 

2014-2018
GPCR-Nanoscreen: Nanoscale High Content Analysis
23 million DKK
Danish Research Councils for Independent and Strategic Research

2013-2017
IBISS: Industrial Biomimetic Sensing and Separation
2.9 million DKK
Danish National Advanced Technology Foundation

2013-2016
PUMPS: Molecular analysis of P-type ATPases from single vesicle to single molecule
1.8 million DKK
Danish Councils for Research and Innovation

2012-2015
Functional Plasicity og G-Protein Coupled Receptor (GPCR) activation og G-protein
1.2 miilon DKK
The Lundbeck Foundation

2014-2017 
"BioSYNergy"UNIK-Synthetic Biology
UCPH Excellence Programme for Interdisciplinary Research, University of Copenhagen 2016 funds and Danish Council for Research and Innovation

People

  • Prof. D. Stamou
  • ~5 postdocs
  • ~5 PhD students
  • 1 administrator